xp 1 400 cell signaling technology 3579 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
xp 1 400 cell signaling technology 3579
Xp 1 400 Cell Signaling Technology 3579, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+sox2/Sox2+XP+Rabbit+mAb/pm41792118-387-250-252
Average 96 stars, based on 942 article reviews
Xp 1 400 Cell Signaling Technology 3579, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+sox2/Sox2+XP+Rabbit+mAb/pm41792118-387-250-252
Average 96 stars, based on 942 article reviews
xp 1 400 cell signaling technology 3579 - by Bioz Stars,
2026-09
96/100 stars
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Software:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Lysis:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Bicinchoninic Acid Protein Assay:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Western Blot:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Imaging:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Reverse Transcription:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Activity Assay:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Immunohistochemistry:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 Polymer:Article Title: Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells Article Snippet: Reagent (in order of appearance in methods) Company and Order Information RPMI 1540 Media Gibco 11875135 DMEM/F12 Media Gibco 11320033 KnockOutTM Serum Replacement Gibco 10828028 Insulin-transferrin-selenium (ITS-G) Gibco 41400045 Ultra-low attachment flat bottom 96-well plates Corning 3474 ImageXpress Molecular Devices ImageXpress Pico CellReporterXpress Software Molecular Devices Version 2.1.5156 Hoechst ThermoFisher Scientific Hoechst 33342 siRNA pools Dharmacon siSOX2 L-011778-00-0005 siNeg D-001810-10-05 Lipofectamine Lipofectamine RNAiMAX ThermoFisher Scientific 13778075 RIPA lysis buffer ThermoFisher Scientific 89901 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail ThermoFisher Scientific 78443 Pierce Rapid Gold BCA Protein Assay Kit ThermoFisher Scientific: A53227 NuPAGE 4-12% Bis-Tris Protein Gels ThermoFisher Scientific NP0322BOX NuPAGE MOPS SDS Running Buffer ThermoFisher Scientific NP0001 Western Blot Antibodies SOX2 Primary: Cell Signaling Technology 3579 GAPDH Primary: MilliporeSigma MAB374 Mouse Secondary HRP-linked: Cell Signaling Technology 34577 Rabbit Secondary HRP-linked: Cell Signaling Technology 7074 SuperSignal Chemiluminescent Substrate ThermoFisher Scientific SuperSignal West PLUS Chemiluminescent Substrate 34577 iBright Imaging System and Software ThermoFisher Scientific iBRIGHT CL1000 Imaging System and Analysis Software NucleoSpin RNA Plus kit Macherey-Nagel 740984.50 Direct-zol RNA Miniprep Plus Kit Zymo Research R2071 SpectraMax QuickDrop Molecular Devices High-Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific 4368814 QuantStudio 3 machine and Design software ThermoFisher Scientific Version 1.5.1 Taqman Probes ThermoFisher Scientific Oct4 Assay ID: Hs04260367_gH Sox2 Assay ID: Hs01053049_s1 Nanog Assay ID: Hs02387400_g1 GAPDH Assay ID: Hs99999905_m1 CD44 Assay ID: Hs01075862_m1 CD117 Assay ID: Hs00174029_m1 CD133 Assay ID: Hs01009259_m1 ALDH1A1 Assay ID: Hs00946916_m1 ALDH1A2 Assay ID: Hs00180254_m1 CellTiter-Glo luminescent reagent Promega G7570 SpectraMax iD3 plate reader Molecular Devices SoftMax Pro Software Molecular Devices Version 7.1.0 CellStripper Corning 25-056-CI Fluorescently-conjugated antibodies from Miltenyi Biotec CD44-FITC 1:100 (#130-113-341) CD117-APC 1:100 (#130-111-671) CD133-FITC 1:100 (#130-110-968) CD133-APC 1:50 (#130-111-080) ALDH Activity Assay AldeRed (Millipore Sigma scr150) AldeFluor (Stem Cell Technologies 01700) BDFACS Melody cell sorter and BDFACS Chorus Software Becton Dickinson FlowJo Becton Dickinson FlowJo Version 10.7.1 Matrigel Corning Matrigel 354263 Lot number 8260015 |